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srp 501 micro bio spintm chromatography columns bio rad
Srp 501 Micro Bio Spintm Chromatography Columns Bio Rad, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 567 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 567 article reviews
Srp 501 Micro Bio Spintm Chromatography Columns Bio Rad, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 567 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/srp+501+micro+bio+spintm+chromatography+columns+bio+rad/Micro+Bio-Spin+Chromatography+Column/pm38776225-521-227-232
Average 99 stars, based on 567 article reviews
srp 501 micro bio spintm chromatography columns bio rad - by Bioz Stars,
2026-09
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Polymerase Chain Reaction:Article Title: The C-terminal disordered loop domain of Apc8 unlocks APC/C mitotic activation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-Xe-Apc1 Lab stock #4853 via BioGenes Rabbit polyclonal anti-Xe-Apc2 (for Xe-Apc2 detection) Lab stock #7577 via BioGenes Rabbit polyclonal anti-Xe-Apc2 (for Xe-Apc2 depletion) Lab stock #7576 via BioGenes Mouse monoclonal anti-Cdc27/Apc3 BD Biosciences Cat#610455; RRID:AB_397828 Mouse monoclonal anti-Apc3 (AF3.1) Gift from J. Gannon N/A Rabbit polyclonal anti-Xe-Apc5 Lab stock #3445 via BioGenes Rabbit polyclonal anti-Xe-Apc6 Lab stock #3446 via BioGenes Rabbit polyclonal anti-Xe-Apc8 Lab stock #3492 via BioGenes anti-HsApc8 Abcam Cat#ab72206; RRID:AB_1268025 Mouse monoclonal anti-Cdc20 (BA8.1) Gift from J. Gannon N/A Mouse monoclonal anti-Cyclin B (X121) Gift from J. Gannon N/A Mouse monoclonal anti-Cdk1(A17) Gift from J. Gannon N/A Mouse monoclonal anti-phosphorylated Plx1 (AZ44) Gift from J. Gannon N/A anti-Xe-Cks2 Lab stock CE/R2 Mouse monoclonal anti-MBP (R29) Gift from J. Gannon N/A anti-ALFA NanoTag Cat#N1582; RRID:AB_3065196 anti-Tubulin Sigma Cat#T9026; RRID:AB_477593 Rabbit polyclonal anti-Xe-Apc1-pS314pS318 Lab stock #25312 via BioGenes Rabbit polyclonal anti-Xe-Apc1-pS358 Lab stock #27644 via BioGenes Rabbit polyclonal anti-Xe-Apc1-pT378pS380 Lab stock #25316 via BioGenes Bacterial and virus strains Escherichia coli DH5a Lab stock N/A Escherichia coli BL21 CodonPlus (DE3)-RIL Agilent 230245 Escherichia coli DH10a Lab stock N/A Multibac baculovirus/insect cell system Zhang Z. et al.43 and Fujimitsu K. et al.21 N/A Chemicals, peptides, and recombinant proteins Cycloheximide (CHX) Sigma-Aldrich Cat#C7698 Okadaic acid (OA) Sigma-Aldrich Cat#O4511 Bis(sulfosuccinimidyl)suberate (BS3) crosslinker Thermo Fisher Scientific Cat#A39266 Recombinant Xe-APC/Cs (WT and mutants) This paper N/A GST-CyclinBD167 Gift from J. Gannon pGEX Hs cyclin BD167 MBP-Apc8 loop fragments (WT and mutants) This paper N/A 10His-Xe-p9/Cks2 Lab stock N/A MBP-ENSA-6His Lab stock N/A Casein Sigma-Aldrich Cat#C8032 CDK2-Cyclin A Gift from J. Gannon N/A Plx1 Lab stock N/A HRV-3C PreScission protease Lab stock N/A Critical commercial assays USER Cloning kit NEB Cat#M5505 TNT T7 Quick coupled transcription/translation system Promega L1170 Pierce ECL Western blotting substrate Thermo Fisher Scientific 32106 (Continued on next page) 16 Cell Reports 43, 114262, June 25, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Crosslinking identification PRIDE PXD050097 Experimental models: Cell lines Spodoptera frugiperda (Sf9) insect cells Gibco B82501 Trichopulsia ni (High Five) insect cells Invitrogen B85502 Experimental models: Organisms/strains Xenopus laevis female NASCO N/A Oligonucleotides Oligonucleotide PCR primers used to generate APC/C loop deletion mutants (sequences available on request) IDT N/A Oligonucleotides PCR primers used to generate Apc8 loop fragments (sequences available on request) IDT N/A Recombinant DNA pFUBB-Apc2/Apc11-Apc1/Apc10 (WT and mutants) This paper N/A pFUBB-Apc3/Apc8/Apc7-Apc5/Apc4/Apc6-StrepII (WT and mutants) This paper N/A pUUBB-Apc13-Apc12/Apc15/Apc16 (WT) Fujimitsu K. et al.21 N/A pHY22-CycB (for IVT substrate) Lab stock Plasmid 372 pHY22-CycBD67 (for IVT substrate) Lab stock Plasmid 373 pHY22-Cdc20-5A Lab stock Plasmid 1017 pET16b-MBP-XeApc8_540-580-6His This paper N/A pET16b-MBP-XeApc8_540–580_4A-6His This paper N/A pET16b-MBP-XeApc8_540–580_8A-6His This paper N/A pET16b-MBP-XeApc8_540–580_S544A-6His This paper N/A pET16b-MBP-XeApc8_540–580_T565A-6His This paper N/A pET16b-MBP-XeApc8_540–580_S571A-6His This paper N/A pET16b-MBP-XeApc8_540–580_T579A-6His This paper N/A pET16b-MBP-XeApc8_540–580_S544A/S571A-6His This paper N/A pET16b-MBP-XeApc8_540–580_T565A/T579A-6His This paper N/A Software and algorithms GraphPad Prism (v10.2.2) GraphPad: https://www.graphpad.com/ RRID:SCR_002798 ImageJ National Institutes of Health RRID:SCR_003070 ImageStudio Lite LI-COR: https://www.licor.com/ bio/image-studio/ RRID:SCR_013715 Adobe Illustrator Adobe: http://www.adobe.com/ products/illustrator.htm RRID:SCR_010279 AlphaFold2 DeepMind: AlphaFold2.ipynb - Colaboratory N/A PyMOL (v2.0.4) Schrödinger: https://pymol.org/2/ RRID:SCR_000305 SnapGene (v4.3.11) Dotmatics: https://www.snapgene.com/ RRID:SCR_015052 PhosphoSitePlus https://www.phosphosite.org RRID:SCR_001837 ProteoWizard (v3.0) ProteoWizard: https://proteowizard. sourceforge.io/ RRID:SCR_012056 Xi software (version 1.7.6.4) Juri Rappsilber Lab44 https://www.rappsilberlab.org/ software/ Other [S35]Met-label, 1000 Ci/mmol, 10 mCi/mL Hartmann Analytic SCIS-103 [g-P32]ATP, 6000 Ci/mmol, 10 mCi/mL Hartmann Analytic Recombinant:Article Title: The C-terminal disordered loop domain of Apc8 unlocks APC/C mitotic activation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-Xe-Apc1 Lab stock #4853 via BioGenes Rabbit polyclonal anti-Xe-Apc2 (for Xe-Apc2 detection) Lab stock #7577 via BioGenes Rabbit polyclonal anti-Xe-Apc2 (for Xe-Apc2 depletion) Lab stock #7576 via BioGenes Mouse monoclonal anti-Cdc27/Apc3 BD Biosciences Cat#610455; RRID:AB_397828 Mouse monoclonal anti-Apc3 (AF3.1) Gift from J. Gannon N/A Rabbit polyclonal anti-Xe-Apc5 Lab stock #3445 via BioGenes Rabbit polyclonal anti-Xe-Apc6 Lab stock #3446 via BioGenes Rabbit polyclonal anti-Xe-Apc8 Lab stock #3492 via BioGenes anti-HsApc8 Abcam Cat#ab72206; RRID:AB_1268025 Mouse monoclonal anti-Cdc20 (BA8.1) Gift from J. Gannon N/A Mouse monoclonal anti-Cyclin B (X121) Gift from J. Gannon N/A Mouse monoclonal anti-Cdk1(A17) Gift from J. Gannon N/A Mouse monoclonal anti-phosphorylated Plx1 (AZ44) Gift from J. Gannon N/A anti-Xe-Cks2 Lab stock CE/R2 Mouse monoclonal anti-MBP (R29) Gift from J. Gannon N/A anti-ALFA NanoTag Cat#N1582; RRID:AB_3065196 anti-Tubulin Sigma Cat#T9026; RRID:AB_477593 Rabbit polyclonal anti-Xe-Apc1-pS314pS318 Lab stock #25312 via BioGenes Rabbit polyclonal anti-Xe-Apc1-pS358 Lab stock #27644 via BioGenes Rabbit polyclonal anti-Xe-Apc1-pT378pS380 Lab stock #25316 via BioGenes Bacterial and virus strains Escherichia coli DH5a Lab stock N/A Escherichia coli BL21 CodonPlus (DE3)-RIL Agilent 230245 Escherichia coli DH10a Lab stock N/A Multibac baculovirus/insect cell system Zhang Z. et al.43 and Fujimitsu K. et al.21 N/A Chemicals, peptides, and recombinant proteins Cycloheximide (CHX) Sigma-Aldrich Cat#C7698 Okadaic acid (OA) Sigma-Aldrich Cat#O4511 Bis(sulfosuccinimidyl)suberate (BS3) crosslinker Thermo Fisher Scientific Cat#A39266 Recombinant Xe-APC/Cs (WT and mutants) This paper N/A GST-CyclinBD167 Gift from J. Gannon pGEX Hs cyclin BD167 MBP-Apc8 loop fragments (WT and mutants) This paper N/A 10His-Xe-p9/Cks2 Lab stock N/A MBP-ENSA-6His Lab stock N/A Casein Sigma-Aldrich Cat#C8032 CDK2-Cyclin A Gift from J. Gannon N/A Plx1 Lab stock N/A HRV-3C PreScission protease Lab stock N/A Critical commercial assays USER Cloning kit NEB Cat#M5505 TNT T7 Quick coupled transcription/translation system Promega L1170 Pierce ECL Western blotting substrate Thermo Fisher Scientific 32106 (Continued on next page) 16 Cell Reports 43, 114262, June 25, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Crosslinking identification PRIDE PXD050097 Experimental models: Cell lines Spodoptera frugiperda (Sf9) insect cells Gibco B82501 Trichopulsia ni (High Five) insect cells Invitrogen B85502 Experimental models: Organisms/strains Xenopus laevis female NASCO N/A Oligonucleotides Oligonucleotide PCR primers used to generate APC/C loop deletion mutants (sequences available on request) IDT N/A Oligonucleotides PCR primers used to generate Apc8 loop fragments (sequences available on request) IDT N/A Recombinant DNA pFUBB-Apc2/Apc11-Apc1/Apc10 (WT and mutants) This paper N/A pFUBB-Apc3/Apc8/Apc7-Apc5/Apc4/Apc6-StrepII (WT and mutants) This paper N/A pUUBB-Apc13-Apc12/Apc15/Apc16 (WT) Fujimitsu K. et al.21 N/A pHY22-CycB (for IVT substrate) Lab stock Plasmid 372 pHY22-CycBD67 (for IVT substrate) Lab stock Plasmid 373 pHY22-Cdc20-5A Lab stock Plasmid 1017 pET16b-MBP-XeApc8_540-580-6His This paper N/A pET16b-MBP-XeApc8_540–580_4A-6His This paper N/A pET16b-MBP-XeApc8_540–580_8A-6His This paper N/A pET16b-MBP-XeApc8_540–580_S544A-6His This paper N/A pET16b-MBP-XeApc8_540–580_T565A-6His This paper N/A pET16b-MBP-XeApc8_540–580_S571A-6His This paper N/A pET16b-MBP-XeApc8_540–580_T579A-6His This paper N/A pET16b-MBP-XeApc8_540–580_S544A/S571A-6His This paper N/A pET16b-MBP-XeApc8_540–580_T565A/T579A-6His This paper N/A Software and algorithms GraphPad Prism (v10.2.2) GraphPad: https://www.graphpad.com/ RRID:SCR_002798 ImageJ National Institutes of Health RRID:SCR_003070 ImageStudio Lite LI-COR: https://www.licor.com/ bio/image-studio/ RRID:SCR_013715 Adobe Illustrator Adobe: http://www.adobe.com/ products/illustrator.htm RRID:SCR_010279 AlphaFold2 DeepMind: AlphaFold2.ipynb - Colaboratory N/A PyMOL (v2.0.4) Schrödinger: https://pymol.org/2/ RRID:SCR_000305 SnapGene (v4.3.11) Dotmatics: https://www.snapgene.com/ RRID:SCR_015052 PhosphoSitePlus https://www.phosphosite.org RRID:SCR_001837 ProteoWizard (v3.0) ProteoWizard: https://proteowizard. sourceforge.io/ RRID:SCR_012056 Xi software (version 1.7.6.4) Juri Rappsilber Lab44 https://www.rappsilberlab.org/ software/ Other [S35]Met-label, 1000 Ci/mmol, 10 mCi/mL Hartmann Analytic SCIS-103 [g-P32]ATP, 6000 Ci/mmol, 10 mCi/mL Hartmann Analytic Plasmid Preparation:Article Title: The C-terminal disordered loop domain of Apc8 unlocks APC/C mitotic activation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-Xe-Apc1 Lab stock #4853 via BioGenes Rabbit polyclonal anti-Xe-Apc2 (for Xe-Apc2 detection) Lab stock #7577 via BioGenes Rabbit polyclonal anti-Xe-Apc2 (for Xe-Apc2 depletion) Lab stock #7576 via BioGenes Mouse monoclonal anti-Cdc27/Apc3 BD Biosciences Cat#610455; RRID:AB_397828 Mouse monoclonal anti-Apc3 (AF3.1) Gift from J. Gannon N/A Rabbit polyclonal anti-Xe-Apc5 Lab stock #3445 via BioGenes Rabbit polyclonal anti-Xe-Apc6 Lab stock #3446 via BioGenes Rabbit polyclonal anti-Xe-Apc8 Lab stock #3492 via BioGenes anti-HsApc8 Abcam Cat#ab72206; RRID:AB_1268025 Mouse monoclonal anti-Cdc20 (BA8.1) Gift from J. Gannon N/A Mouse monoclonal anti-Cyclin B (X121) Gift from J. Gannon N/A Mouse monoclonal anti-Cdk1(A17) Gift from J. Gannon N/A Mouse monoclonal anti-phosphorylated Plx1 (AZ44) Gift from J. Gannon N/A anti-Xe-Cks2 Lab stock CE/R2 Mouse monoclonal anti-MBP (R29) Gift from J. Gannon N/A anti-ALFA NanoTag Cat#N1582; RRID:AB_3065196 anti-Tubulin Sigma Cat#T9026; RRID:AB_477593 Rabbit polyclonal anti-Xe-Apc1-pS314pS318 Lab stock #25312 via BioGenes Rabbit polyclonal anti-Xe-Apc1-pS358 Lab stock #27644 via BioGenes Rabbit polyclonal anti-Xe-Apc1-pT378pS380 Lab stock #25316 via BioGenes Bacterial and virus strains Escherichia coli DH5a Lab stock N/A Escherichia coli BL21 CodonPlus (DE3)-RIL Agilent 230245 Escherichia coli DH10a Lab stock N/A Multibac baculovirus/insect cell system Zhang Z. et al.43 and Fujimitsu K. et al.21 N/A Chemicals, peptides, and recombinant proteins Cycloheximide (CHX) Sigma-Aldrich Cat#C7698 Okadaic acid (OA) Sigma-Aldrich Cat#O4511 Bis(sulfosuccinimidyl)suberate (BS3) crosslinker Thermo Fisher Scientific Cat#A39266 Recombinant Xe-APC/Cs (WT and mutants) This paper N/A GST-CyclinBD167 Gift from J. Gannon pGEX Hs cyclin BD167 MBP-Apc8 loop fragments (WT and mutants) This paper N/A 10His-Xe-p9/Cks2 Lab stock N/A MBP-ENSA-6His Lab stock N/A Casein Sigma-Aldrich Cat#C8032 CDK2-Cyclin A Gift from J. Gannon N/A Plx1 Lab stock N/A HRV-3C PreScission protease Lab stock N/A Critical commercial assays USER Cloning kit NEB Cat#M5505 TNT T7 Quick coupled transcription/translation system Promega L1170 Pierce ECL Western blotting substrate Thermo Fisher Scientific 32106 (Continued on next page) 16 Cell Reports 43, 114262, June 25, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Crosslinking identification PRIDE PXD050097 Experimental models: Cell lines Spodoptera frugiperda (Sf9) insect cells Gibco B82501 Trichopulsia ni (High Five) insect cells Invitrogen B85502 Experimental models: Organisms/strains Xenopus laevis female NASCO N/A Oligonucleotides Oligonucleotide PCR primers used to generate APC/C loop deletion mutants (sequences available on request) IDT N/A Oligonucleotides PCR primers used to generate Apc8 loop fragments (sequences available on request) IDT N/A Recombinant DNA pFUBB-Apc2/Apc11-Apc1/Apc10 (WT and mutants) This paper N/A pFUBB-Apc3/Apc8/Apc7-Apc5/Apc4/Apc6-StrepII (WT and mutants) This paper N/A pUUBB-Apc13-Apc12/Apc15/Apc16 (WT) Fujimitsu K. et al.21 N/A pHY22-CycB (for IVT substrate) Lab stock Plasmid 372 pHY22-CycBD67 (for IVT substrate) Lab stock Plasmid 373 pHY22-Cdc20-5A Lab stock Plasmid 1017 pET16b-MBP-XeApc8_540-580-6His This paper N/A pET16b-MBP-XeApc8_540–580_4A-6His This paper N/A pET16b-MBP-XeApc8_540–580_8A-6His This paper N/A pET16b-MBP-XeApc8_540–580_S544A-6His This paper N/A pET16b-MBP-XeApc8_540–580_T565A-6His This paper N/A pET16b-MBP-XeApc8_540–580_S571A-6His This paper N/A pET16b-MBP-XeApc8_540–580_T579A-6His This paper N/A pET16b-MBP-XeApc8_540–580_S544A/S571A-6His This paper N/A pET16b-MBP-XeApc8_540–580_T565A/T579A-6His This paper N/A Software and algorithms GraphPad Prism (v10.2.2) GraphPad: https://www.graphpad.com/ RRID:SCR_002798 ImageJ National Institutes of Health RRID:SCR_003070 ImageStudio Lite LI-COR: https://www.licor.com/ bio/image-studio/ RRID:SCR_013715 Adobe Illustrator Adobe: http://www.adobe.com/ products/illustrator.htm RRID:SCR_010279 AlphaFold2 DeepMind: AlphaFold2.ipynb - Colaboratory N/A PyMOL (v2.0.4) Schrödinger: https://pymol.org/2/ RRID:SCR_000305 SnapGene (v4.3.11) Dotmatics: https://www.snapgene.com/ RRID:SCR_015052 PhosphoSitePlus https://www.phosphosite.org RRID:SCR_001837 ProteoWizard (v3.0) ProteoWizard: https://proteowizard. sourceforge.io/ RRID:SCR_012056 Xi software (version 1.7.6.4) Juri Rappsilber Lab44 https://www.rappsilberlab.org/ software/ Other [S35]Met-label, 1000 Ci/mmol, 10 mCi/mL Hartmann Analytic SCIS-103 [g-P32]ATP, 6000 Ci/mmol, 10 mCi/mL Hartmann Analytic Software:Article Title: The C-terminal disordered loop domain of Apc8 unlocks APC/C mitotic activation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-Xe-Apc1 Lab stock #4853 via BioGenes Rabbit polyclonal anti-Xe-Apc2 (for Xe-Apc2 detection) Lab stock #7577 via BioGenes Rabbit polyclonal anti-Xe-Apc2 (for Xe-Apc2 depletion) Lab stock #7576 via BioGenes Mouse monoclonal anti-Cdc27/Apc3 BD Biosciences Cat#610455; RRID:AB_397828 Mouse monoclonal anti-Apc3 (AF3.1) Gift from J. Gannon N/A Rabbit polyclonal anti-Xe-Apc5 Lab stock #3445 via BioGenes Rabbit polyclonal anti-Xe-Apc6 Lab stock #3446 via BioGenes Rabbit polyclonal anti-Xe-Apc8 Lab stock #3492 via BioGenes anti-HsApc8 Abcam Cat#ab72206; RRID:AB_1268025 Mouse monoclonal anti-Cdc20 (BA8.1) Gift from J. Gannon N/A Mouse monoclonal anti-Cyclin B (X121) Gift from J. Gannon N/A Mouse monoclonal anti-Cdk1(A17) Gift from J. Gannon N/A Mouse monoclonal anti-phosphorylated Plx1 (AZ44) Gift from J. Gannon N/A anti-Xe-Cks2 Lab stock CE/R2 Mouse monoclonal anti-MBP (R29) Gift from J. Gannon N/A anti-ALFA NanoTag Cat#N1582; RRID:AB_3065196 anti-Tubulin Sigma Cat#T9026; RRID:AB_477593 Rabbit polyclonal anti-Xe-Apc1-pS314pS318 Lab stock #25312 via BioGenes Rabbit polyclonal anti-Xe-Apc1-pS358 Lab stock #27644 via BioGenes Rabbit polyclonal anti-Xe-Apc1-pT378pS380 Lab stock #25316 via BioGenes Bacterial and virus strains Escherichia coli DH5a Lab stock N/A Escherichia coli BL21 CodonPlus (DE3)-RIL Agilent 230245 Escherichia coli DH10a Lab stock N/A Multibac baculovirus/insect cell system Zhang Z. et al.43 and Fujimitsu K. et al.21 N/A Chemicals, peptides, and recombinant proteins Cycloheximide (CHX) Sigma-Aldrich Cat#C7698 Okadaic acid (OA) Sigma-Aldrich Cat#O4511 Bis(sulfosuccinimidyl)suberate (BS3) crosslinker Thermo Fisher Scientific Cat#A39266 Recombinant Xe-APC/Cs (WT and mutants) This paper N/A GST-CyclinBD167 Gift from J. Gannon pGEX Hs cyclin BD167 MBP-Apc8 loop fragments (WT and mutants) This paper N/A 10His-Xe-p9/Cks2 Lab stock N/A MBP-ENSA-6His Lab stock N/A Casein Sigma-Aldrich Cat#C8032 CDK2-Cyclin A Gift from J. Gannon N/A Plx1 Lab stock N/A HRV-3C PreScission protease Lab stock N/A Critical commercial assays USER Cloning kit NEB Cat#M5505 TNT T7 Quick coupled transcription/translation system Promega L1170 Pierce ECL Western blotting substrate Thermo Fisher Scientific 32106 (Continued on next page) 16 Cell Reports 43, 114262, June 25, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Crosslinking identification PRIDE PXD050097 Experimental models: Cell lines Spodoptera frugiperda (Sf9) insect cells Gibco B82501 Trichopulsia ni (High Five) insect cells Invitrogen B85502 Experimental models: Organisms/strains Xenopus laevis female NASCO N/A Oligonucleotides Oligonucleotide PCR primers used to generate APC/C loop deletion mutants (sequences available on request) IDT N/A Oligonucleotides PCR primers used to generate Apc8 loop fragments (sequences available on request) IDT N/A Recombinant DNA pFUBB-Apc2/Apc11-Apc1/Apc10 (WT and mutants) This paper N/A pFUBB-Apc3/Apc8/Apc7-Apc5/Apc4/Apc6-StrepII (WT and mutants) This paper N/A pUUBB-Apc13-Apc12/Apc15/Apc16 (WT) Fujimitsu K. et al.21 N/A pHY22-CycB (for IVT substrate) Lab stock Plasmid 372 pHY22-CycBD67 (for IVT substrate) Lab stock Plasmid 373 pHY22-Cdc20-5A Lab stock Plasmid 1017 pET16b-MBP-XeApc8_540-580-6His This paper N/A pET16b-MBP-XeApc8_540–580_4A-6His This paper N/A pET16b-MBP-XeApc8_540–580_8A-6His This paper N/A pET16b-MBP-XeApc8_540–580_S544A-6His This paper N/A pET16b-MBP-XeApc8_540–580_T565A-6His This paper N/A pET16b-MBP-XeApc8_540–580_S571A-6His This paper N/A pET16b-MBP-XeApc8_540–580_T579A-6His This paper N/A pET16b-MBP-XeApc8_540–580_S544A/S571A-6His This paper N/A pET16b-MBP-XeApc8_540–580_T565A/T579A-6His This paper N/A Software and algorithms GraphPad Prism (v10.2.2) GraphPad: https://www.graphpad.com/ RRID:SCR_002798 ImageJ National Institutes of Health RRID:SCR_003070 ImageStudio Lite LI-COR: https://www.licor.com/ bio/image-studio/ RRID:SCR_013715 Adobe Illustrator Adobe: http://www.adobe.com/ products/illustrator.htm RRID:SCR_010279 AlphaFold2 DeepMind: AlphaFold2.ipynb - Colaboratory N/A PyMOL (v2.0.4) Schrödinger: https://pymol.org/2/ RRID:SCR_000305 SnapGene (v4.3.11) Dotmatics: https://www.snapgene.com/ RRID:SCR_015052 PhosphoSitePlus https://www.phosphosite.org RRID:SCR_001837 ProteoWizard (v3.0) ProteoWizard: https://proteowizard. sourceforge.io/ RRID:SCR_012056 Xi software (version 1.7.6.4) Juri Rappsilber Lab44 https://www.rappsilberlab.org/ software/ Other [S35]Met-label, 1000 Ci/mmol, 10 mCi/mL Hartmann Analytic SCIS-103 [g-P32]ATP, 6000 Ci/mmol, 10 mCi/mL Hartmann Analytic Chromatography:Article Title: The C-terminal disordered loop domain of Apc8 unlocks APC/C mitotic activation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-Xe-Apc1 Lab stock #4853 via BioGenes Rabbit polyclonal anti-Xe-Apc2 (for Xe-Apc2 detection) Lab stock #7577 via BioGenes Rabbit polyclonal anti-Xe-Apc2 (for Xe-Apc2 depletion) Lab stock #7576 via BioGenes Mouse monoclonal anti-Cdc27/Apc3 BD Biosciences Cat#610455; RRID:AB_397828 Mouse monoclonal anti-Apc3 (AF3.1) Gift from J. Gannon N/A Rabbit polyclonal anti-Xe-Apc5 Lab stock #3445 via BioGenes Rabbit polyclonal anti-Xe-Apc6 Lab stock #3446 via BioGenes Rabbit polyclonal anti-Xe-Apc8 Lab stock #3492 via BioGenes anti-HsApc8 Abcam Cat#ab72206; RRID:AB_1268025 Mouse monoclonal anti-Cdc20 (BA8.1) Gift from J. Gannon N/A Mouse monoclonal anti-Cyclin B (X121) Gift from J. Gannon N/A Mouse monoclonal anti-Cdk1(A17) Gift from J. Gannon N/A Mouse monoclonal anti-phosphorylated Plx1 (AZ44) Gift from J. Gannon N/A anti-Xe-Cks2 Lab stock CE/R2 Mouse monoclonal anti-MBP (R29) Gift from J. Gannon N/A anti-ALFA NanoTag Cat#N1582; RRID:AB_3065196 anti-Tubulin Sigma Cat#T9026; RRID:AB_477593 Rabbit polyclonal anti-Xe-Apc1-pS314pS318 Lab stock #25312 via BioGenes Rabbit polyclonal anti-Xe-Apc1-pS358 Lab stock #27644 via BioGenes Rabbit polyclonal anti-Xe-Apc1-pT378pS380 Lab stock #25316 via BioGenes Bacterial and virus strains Escherichia coli DH5a Lab stock N/A Escherichia coli BL21 CodonPlus (DE3)-RIL Agilent 230245 Escherichia coli DH10a Lab stock N/A Multibac baculovirus/insect cell system Zhang Z. et al.43 and Fujimitsu K. et al.21 N/A Chemicals, peptides, and recombinant proteins Cycloheximide (CHX) Sigma-Aldrich Cat#C7698 Okadaic acid (OA) Sigma-Aldrich Cat#O4511 Bis(sulfosuccinimidyl)suberate (BS3) crosslinker Thermo Fisher Scientific Cat#A39266 Recombinant Xe-APC/Cs (WT and mutants) This paper N/A GST-CyclinBD167 Gift from J. Gannon pGEX Hs cyclin BD167 MBP-Apc8 loop fragments (WT and mutants) This paper N/A 10His-Xe-p9/Cks2 Lab stock N/A MBP-ENSA-6His Lab stock N/A Casein Sigma-Aldrich Cat#C8032 CDK2-Cyclin A Gift from J. Gannon N/A Plx1 Lab stock N/A HRV-3C PreScission protease Lab stock N/A Critical commercial assays USER Cloning kit NEB Cat#M5505 TNT T7 Quick coupled transcription/translation system Promega L1170 Pierce ECL Western blotting substrate Thermo Fisher Scientific 32106 (Continued on next page) 16 Cell Reports 43, 114262, June 25, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Crosslinking identification PRIDE PXD050097 Experimental models: Cell lines Spodoptera frugiperda (Sf9) insect cells Gibco B82501 Trichopulsia ni (High Five) insect cells Invitrogen B85502 Experimental models: Organisms/strains Xenopus laevis female NASCO N/A Oligonucleotides Oligonucleotide PCR primers used to generate APC/C loop deletion mutants (sequences available on request) IDT N/A Oligonucleotides PCR primers used to generate Apc8 loop fragments (sequences available on request) IDT N/A Recombinant DNA pFUBB-Apc2/Apc11-Apc1/Apc10 (WT and mutants) This paper N/A pFUBB-Apc3/Apc8/Apc7-Apc5/Apc4/Apc6-StrepII (WT and mutants) This paper N/A pUUBB-Apc13-Apc12/Apc15/Apc16 (WT) Fujimitsu K. et al.21 N/A pHY22-CycB (for IVT substrate) Lab stock Plasmid 372 pHY22-CycBD67 (for IVT substrate) Lab stock Plasmid 373 pHY22-Cdc20-5A Lab stock Plasmid 1017 pET16b-MBP-XeApc8_540-580-6His This paper N/A pET16b-MBP-XeApc8_540–580_4A-6His This paper N/A pET16b-MBP-XeApc8_540–580_8A-6His This paper N/A pET16b-MBP-XeApc8_540–580_S544A-6His This paper N/A pET16b-MBP-XeApc8_540–580_T565A-6His This paper N/A pET16b-MBP-XeApc8_540–580_S571A-6His This paper N/A pET16b-MBP-XeApc8_540–580_T579A-6His This paper N/A pET16b-MBP-XeApc8_540–580_S544A/S571A-6His This paper N/A pET16b-MBP-XeApc8_540–580_T565A/T579A-6His This paper N/A Software and algorithms GraphPad Prism (v10.2.2) GraphPad: https://www.graphpad.com/ RRID:SCR_002798 ImageJ National Institutes of Health RRID:SCR_003070 ImageStudio Lite LI-COR: https://www.licor.com/ bio/image-studio/ RRID:SCR_013715 Adobe Illustrator Adobe: http://www.adobe.com/ products/illustrator.htm RRID:SCR_010279 AlphaFold2 DeepMind: AlphaFold2.ipynb - Colaboratory N/A PyMOL (v2.0.4) Schrödinger: https://pymol.org/2/ RRID:SCR_000305 SnapGene (v4.3.11) Dotmatics: https://www.snapgene.com/ RRID:SCR_015052 PhosphoSitePlus https://www.phosphosite.org RRID:SCR_001837 ProteoWizard (v3.0) ProteoWizard: https://proteowizard. sourceforge.io/ RRID:SCR_012056 Xi software (version 1.7.6.4) Juri Rappsilber Lab44 https://www.rappsilberlab.org/ software/ Other [S35]Met-label, 1000 Ci/mmol, 10 mCi/mL Hartmann Analytic SCIS-103 [g-P32]ATP, 6000 Ci/mmol, 10 mCi/mL Hartmann Analytic |